Directed Enzyme Evolution: Screening and Selection Methods

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Directed evolution comprises two distinct steps that are typically applied in an iterative fashion: (1) generating molecular diversity and (2) finding among the ensemble of mutant sequences those proteins that perform the desired fu- tion according to the specified criteria. In many ways, the second step is the most challenging. No matter how cleverly designed or diverse the starting library, without an effective screening strategy the ability to isolate useful clones is severely diminished. The best screens are (1) high ...

Directed Enzyme Evolution: Screening and Selection Methods 2010, Humana Press Inc., Totowa, NJ

ISBN-13: 9781617374722

Paperback

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Directed Enzyme Evolution: Screening and Selection Methods 2003, Humana Press

ISBN-13: 9781588292865

2003 edition

Hardcover

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