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Bourbaki Gambit
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Carl Djerassi
At the age of 68, distinguished Princeton science professor Max Weiss is bribed into taking an early retirement. He takes an ingenious revenge in the form of "Doctor Diana Skordylis"--a pseudonym for a partnership among Weiss and three aging colleagues. Their soaring success is unanticipated and professional jealousy soon threatens Diana Skordylis ...
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PCR Primer Design
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Anton Yuryev (Editor)
In the past decade, molecular biology has been transformed from the art of cloning a single gene to a statistical science measuring and calculating properties of entire genomes. New high-throughput methods have been developed for genome sequencing and studying the cell at different systematic levels such as transcriptome, proteome, metabolome and ...
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PCR in situ hybridization protocols and applications
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Nuovo
Written by a pathologist noted for his work on molecular techniques for detecting human papillomarivus infection, this volume explains the principles, methodology, and diagnostic applications of PCR in situ hybridization. Because this technique combines the sensitivity of the polymerase chain reaction (PCR) with the cell localizing ability of in ...
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Making PCR: A Story of Biotechnology
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by
Paul Rabinow
This is a behind-the-scenes account of the invention of one of the most significant biotechnological discoveries in our time - the polymerase chain reaction. Transforming the practice and potential of molecular biology, PCR extends scientists' ability to identify and manipulate genetic materials and accurately reproduces millions of copies of a ...
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Molecular Analysis and Genome Discovery
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Stuart Harbron (Editor), Ralph Rapley (Editor)
This advanced level textbook provides a comprehensive overview of recent developments in the area of molecular based diagnostics (including nucleic acids, biosensors and immunoessays) of disease markers. It also covers the impact of techniques such as in vitro nucleic acid amplifications (e.g. PCR) and other amplification methods, as well as gene ...
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Prins and in Situ PCR Protocols
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Franck Pellestor (Editor)
This completely revised and updated edition takes advantage of the many new developments and applications that have occurred in PRINS and in situ PCR technology, including a new fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutation analysis, a new dideoxy PRINS ...
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Clinical Applications of PCR
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Y M Dennis Lo (Editor)
This book presents an unprecedented collection of core PCR techniques useful for the study and diagnosis of human diseases. Cutting-edge and essential for today's diagnostic laboratories, these techniques heavily utilize nonisotopic, solution phase, and in situ amplification methods. A significant number of chapters describe applications ...
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Morphology Methods
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Ricardo V Lloyd (Editor)
Ricardo Lloyd, md, phd, provides critical and tutorial surveys of the cutting-edge molecular and cell biologic techniques used today in the analysis of cells and tissues in situ. These molecular-based histologic and morphologic techniques are described in step-by-step detail by hands-on experts, often those who have originated or perfected them. ...
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Differential Display: A Practical Approach
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Ron A Leslie, H A Robertson (Editor)
One of the most challenging tasks facing the modern biological research laboratory is to make sense of the enormous amount of data being generated by various genome projects currently underway, and especially the human genome project. Understanding the ways in which genes are differentially expressed in various tissues and cell types, throughout ...
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Differential Display Methods and Protocols
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by
Peng Liang (Editor), Arthur B Pardee (Editor)
The second edition of the highly acclaimed Differential Display Methods and Protocols consists of a wider-ranging collection of chapters that highlight both recent methodological refinements and some fine examples of differential display (DD) applications in recent years. Part I covers gene discovery methodologies that have a unique place in the ...
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PCR 3: PCR in Situ Hybridization: A Practical Approach
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by
O'Leary Herrington, C Simon Herrington (Editor), John J O'Leary (Editor)
PCR in situ hybridization allows the detection of specific nucleic acid sequences and their distribution in the cell. It combines two powerful techniquesin situ hybridization (ISH), which allows cellular localization of DNA and RNA sequences in cells and tissues, and the polymerase chain reaction (PCR), which allows reproducible amplification of ...
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PCR Applications: Protocols for Functional Genomics
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by
Michael A Innis (Editor), John J Sninsky (Editor), David Gelfand (Editor)
PCR is the most powerful technique currently used in molecular biology. It enables the scientist to quickly replicate DNA and RNA on the benchtop. From its discovery in the early 80's, PCR has blossomed into a method that enables everything from ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences daily. "PCR ...
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Species Diagnostics Protocols: PCR and Other Nucleic Acid Methods
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by
Justin P Clapp (Editor)
In Species Diagnostics Protocols leading practitioners describe in full detail numerous methods for identifying many organisms by means of nucleic acid analysis. The applications range from medical diagnosis and plant pathology to medical entomology, mycology, and molecular ecology. The organisms identified include plant, insect, and vertebrate ...
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PCR Protocols in Molecular Toxicology
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by
John P Vanden Heuvel, Ph.D. (Editor), John P Vanden Heuvel (Editor)
Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects.PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are ...
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PCR Technology: Current Innovations, Second Edition
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by
Thomas Weissensteiner (Editor), Hugh G Griffin, Annette M Griffin
In the decade since the publication of the bestselling first edition, Polymerase Chain Reaction (PCR) technology has been at the forefront of dramatic developments in biochemistry. Many exciting advances have been made in this specialized field of DNA analysis and a large number of commercial kits and additives are now available. There has never ...
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Nucleic Acid Amplification Technologies: Application to Disease Diagnosis
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by
Helen Lee, Stephen A Morse
Providing current information and guidance on the uses of various nucleic acid amplification technologies for clinical laboratory diagnosis, this volume goes beyond the Polymerase Chain Reaction to explore a broader range of important alternative DNA/RNA amplification methods.
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PCR Cloning Protocols
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by
Bing-Yuan Chen (Editor), Harry W Janes (Editor)
"PCR Cloning Protocols, Second Edition", updates and expands Bruce White's best-selling "PCR Cloning Protocols" (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and ...
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Rapid Detection of Infectious Agents
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by
Stephen Specter, Steven Specter (Editor), Mauro Bendinelli (Editor)
Busy clinicians and health practitioners recognize the importance of speedy detection of pathogens to impede the further spread of infection, and to ensure their patients' rapid and complete recovery. This reader-friendly reference is a unique collection of the newest and most effective diagnostic techniques currently in use in clinical and ...
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A Low-Cost Approach to PCR
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by
Eva Harris
The polymerase chain reaction (PCR) is a technique used to replicate specific pieces of DNA millions of times, which permits the detection and analysis of minute amounts of nucleic acids. Since its introduction in the late 1980s, this technique has been applied not only in molecular biology research but also in fields as diverse as anthropology, ...
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Single Cell Diagnostics: Methods and Protocols
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by
Alan Thornhill (Editor)
The aim of "Single Cell Diagnostics: Methods and Protocols" is for all readers to extend their knowledge and expertise in analysis of single cells. The book starts with laser assisted cell collection, non-invasive assessment of single cells and moves through the techniques of standard fluorescence in situ hybridization and polymerase chain ...
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Making PCR
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by
Paul Rabinow
This study tells the behind-the-scenes story of the invention of the PCR, the polymerase chain reaction. Blending narrative description and interviews with all the major players, Paul Rabinow explores what it means to be a "scientist" today, the effects of carrying out science in the high-risk, high-reward environment of biotech, and what a ...
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Environmental Applications of Nucleic Acid Amplification Technology
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by
Gary A Toranzos, Toranzos A Toranzos
From the Preface Antibody techniques have allowed us to study microorganisms in situ. However, until recently all methodology lacked the sensitivity necessary for environmental work where microorganisms are in most cases present at very low concentrations or where microbial ecosystems contain a myriad of different organisms. Gene probes have been ...
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Nucleic Acid Protocols Handbook
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by
Ralph Rapley (Editor)
This is a comprehensive treasury of all the key molecular biology methods - ranging from DNA extraction to gene localization in situ - needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed "Methods in Molecular Biology" series, providing an introduction to the ...
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In Situ Polymerase Chain Reaction and Related Technology
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by
Jiang Gu (Editor)
"In situ" PCR is capable of detecting minute quantities of DNA or RNA in intact cells or tissues. It provides a useful tool for answering a number of questions that might not otherwise have been investigated. A number of applications of this technology have been reported, bringing advancement in the understanding of many normal and pathalogical ...
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PCR Methods in Foods
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by
John Maurer (Editor), Michael P Doyle (Editor)
This book is a primer for students, faculty, and other professionals interested in molecular biology and its integration into food safety, by introducing the reader to diagnostic PCR-based technologies used in detection of pathogens in foods. This book will enable its reader to: understand the principles behind PCR including real-time; know the ...
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