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Non-Isotopic Methods in Molecular Biology: A Practical Approach
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E Levy (Editor), C S Herrington (Editor)
Radioactive chemicals, whilst playing a vital role in many research and clinical techniques for nucleic acid analysis, are considered dangerous to the laboratory worker and require specialist handling and disposal equipment. This text describes how detailed molecular analysis of DNA and RNA can be performed using a range of non-radioactive ...
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Mol Probes Vol 1 Antibodies (P) 93
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by
Susan Hockfield, S Hockfield et al
A basic manual for individuals beginning to use antibody or nucleic acid probes, providing a sufficient introduction to each technique so that readers may then make use of methods published in journals and other primary sources. Special emphasis is placed on techniques of particular importance to ne
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Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols
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by
Andreas Marx (Editor), Dr. Oliver Seitz (Editor)
Significant DNA sequence data has been made availabe by several important genome projects. We clearly live in an exciting era, in which the allocation of functions to a mere string of the four letters in the code of life plays a major if not the predominant role of ongoing and future research. In functional genomics techniques are required that ...
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Gene Probes
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by
Marilena Aquino de Muro (Editor), Ralph Rapley (Editor), Marilena Aquino de Muro
Senior scientists Marilena Aquino de Muro and Ralph Rapley have brought together an outstanding collection of time-tested protocols for designing and using genes probes in a wide variety of applications. The applications covered range from searching for specific genes in the human chromosome to the detection of microorganisms and their toxins in ...
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In situ hybridization histochemistry
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by
Chesselet
The goal of this fascinating new book is to review the diversity of methods available to apply in situ hybridization histochemistry (ISHH) to a variety of experimental questions. This work includes topics such as synthesis and use of nick-translated DNA probes for ISHH, synthesis and use of oligomeric DNA probes for ISHH, and synthesis and use of ...
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Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols
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by
Vladimir V Didenko (Editor)
This is the first comprehensive treatment of energy transfer (ET) nucleic acid probes. Hands-on experts thoroughly describe all the major probes, both fluorescence resonance energy transfer (FRET)-based and non-FRET-based, and provide a complete set of techniques to monitor DNA and RNA reactions, including hybridization, amplification, cleavage, ...
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Protocols for Nucleic Acid Analysis by Nonradioactive Probes
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Peter G Isaac (Editor)
This book provides an exceptionally useful collection of tried and tested protocols for nucleic acid analysis that avoid the use of radioisotopes. Southern (i.e., DNA) and Northern (i.e., RNA) blotting protocols are covered in detail, from how to isolate good quality nucleic acids from plant and animal sources, to the use of the widest range of ...
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Nucleic Acid Analysis: Principles and Bioapplications
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Charles A Dangler (Editor)
The application of nucleic acid probes is on the threshold of widespread commercial exploitation. Nucleic acid hybridization is one of the most powerful tools used in the laboratory today. For years it has been used to characterize the relationship between known and unknown genetic sequences. However, the numerous possible commercial applications ...
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Nucleic Acid Probes
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by
Robert H Symons, Richard A Watts (Contributions by), Shaker A Mousa (Contributions by)
With each chapter written by a distinguished expert in the biochemistry field, this comprehensive volume describes the preparation and use of a variety of radioactive and non-radioactive probes in situations ranging from research laboratories to routine diagnosis laboratories. The enzymatic and chemical techniques for labeling nucleic acid probes ...
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Nucleic Acid Probes: A Primer for Pathologists
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Margaret Piper
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Visualization of Nucleic Acids
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by
Morel Morel, Sir Gerard Morel (Editor)
This book presents a review of the principle approaches for visualizing DNA and RNA. Using scanning tunneling and atomic force microscopes, the three-dimensional image of the surface of nucleic acids can be seen at atomic-scale resolutions. Spreading methods provide useful details on structural features of isolated molecules, but the major ...
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Molecular methods for virus detection
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by
Danny L Wiedbrauk (Editor), Daniel H Farkas (Editor)
Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods. Written by the inventors or principal developers of these technologies, "Molecular Methods for Virus Detection" ...
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Hybridization with Nucleic Acid Probes
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by
P Tijssen
Recent breakthroughs in recombinant DNA technology and the availability of sophisticated equipment accessible to almost any laboratory, have contributed to the development and perfection of powerful hybridization tools. Recently, nucleic acid hybridization has not only become a cornerstone in molecular biology research but also a powerful ...
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Selected methods for antibody and nucleic acid probes
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by
Hockfield
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Enteric Virus Detection in Water by Nucleic Acid Methods
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by
Mark D Sobsey
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