This historic book may have numerous typos and missing text. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1912 Excerpt: ...amount of citrate must be used in the third bleeding if it is done in less than a week after the former bleeding. It is much better to have ...Read MoreThis historic book may have numerous typos and missing text. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1912 Excerpt: ...amount of citrate must be used in the third bleeding if it is done in less than a week after the former bleeding. It is much better to have at least six large animals for this purpose and to bleed them at long intervals. The culture tissues were removed under strictly aseptic conditions and placed directly into physiological salt solution or Ringer's solution at body temperature. Two types of cultures were made: (1) Cover-glass preparations and (2) watch-glass preparations. The former consisted of pinhead-sized tissues snipped from various organs and placed upon cover slips. These tissues were kept well moistened with salt solution until the plasma was added. As soon as the desired number of preparations were made 0.1 c. c. of the calcium chloride solution was added to 1 c. c. of the citrated plasma and mixed. Small drops of this were placed immediately upon the tissues. This was followed by immediate coagulation of the plasma around the tissue. No calcium was necessary with the 0.5 c. c. citrated plasma, as it coagulated in about 10 minutes after it was dropped on the tissues. The preparations were then inverted and placed over cell slides such as are ordinarily used in bacteriological study or over the usual microscopic slides with metal, glass, or other rings to support the cover slip. The ordinary slide with copper-wire rings was found to be most convenient for this purpose. The rings were first immersed in hot molten paraffin and then placed on the sterile slides. Upon cooling they stuck to the slide, thus forming a larger cell than those used for hanging drop study. The ring slides have this advantage, that the culture does not come into contact with the slide, which often happens in the cell slide and results in the preparation sticking to the slide ...Read Less
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